Mj. Meade et al., OPTIMIZATION OF NOVEL EXTRACELLULAR POLYSACCHARIDE PRODUCTION BY AN ENTEROBACTER SP ON WOOD HYDROLYSATES, Applied and environmental microbiology, 60(4), 1994, pp. 1367-1369
An environmental isolate identified as Enterobacter cloacae has been f
ound to produce a highly viscous, anionic extracellular polysaccharide
(EPS) from a weak mineral acid hydrolysate of hardwood. Production of
this EPS has been optimized on the hydrolysate (initial pH, 6.3; NH4C
l amendment, 0.1%) so that crude yields approaching 9.83 g/liter were
obtained. Although this EPS is polydisperse, its molecular mass as det
ermined by gel exclusion chromatography centers at approximately 1,700
kDa. Solutions of this EPS have been examined rheologically under a v
ariety of conditions and compare favorably with both xanthan and algin
ate.