Bridging of nerve defects with muscle tissue is usually done with deep
frozen grafts. The method has the disadvantage of considerable shrink
age and stiffening of the graft, as well as devitalization of the stru
ctures accompanying muscle fibers. As an alternative method an experim
ent on rats was done to test the use of refrigerated grafts, which do
not have those disadvantages. The morphological evidence was that refr
igerated grafts give as least as good results as deep frozen grafts.