M. Sugihara et al., INDUCTION OF DIFFERENTIATION BY RADIATION AND HYPERTHERMIA IN NEUROBLASTOMA-GLIOMA HYBRID-CELLS, Cell biochemistry and function, 12(2), 1994, pp. 137-142
The effects of either radiation or hyperthermia on the differentiation
potential of NG108-15, a neuroblastoma-glioma hybrid cell line, were
studied. After radiation and hyperthermia, the outgrowth of neurites f
rom NG108-15 cells was potentiated, and polarizing current and voltage
pulses induced a distinct action potential and a diphasic (inward fol
lowing outward) current, respectively. An increase in the specific act
ivity of acetylcholinesterase was also observed. In addition, both tre
atments induced an elevation of the concentration of intracellular cal
cium in some cells. The increase in intracellular calcium concentratio
n caused by applying the calcium ionophore, A23187, induced differenti
ation. It is suggested that both the radiation- and the hyperthermia-i
nduced increases of electrical excitability and acetylcholinesterase a
ctivity may have originated from an increase in intracellular Ca2+ con
centration.