INCREASED ENCAPSULATION AND VIRULENCE OF FRANCISELLA-TULARENSIS LIVE VACCINE STRAIN (LVS) BY SUBCULTURING ON SYNTHETIC MEDIUM

Authors
CHERWONOGRODZKY JW KNODEL MH SPENCE MR
Citation
Jw. Cherwonogrodzky et al., INCREASED ENCAPSULATION AND VIRULENCE OF FRANCISELLA-TULARENSIS LIVE VACCINE STRAIN (LVS) BY SUBCULTURING ON SYNTHETIC MEDIUM, Vaccine, 12(9), 1994, pp. 773-775
Citations number
9
Categorie Soggetti
Immunology
Journal title
Vaccine → ACNP
ISSN journal
0264410X
Volume
12
Issue
9
Year of publication
1994
Pages
773 - 775
Database
ISI
SICI code
0264-410X(1994)12:9<773:IEAVOF>2.0.ZU;2-2
Abstract
Francisella tularensis live vaccine strain (LVS), taken directly from lyophilized vials, did not appear to have a capsule and had low virule nce for balb/c mice. When this strain was subcultured on Chamberlain's synthetic medium (pH 6.5), it became extensively capsulated (1-5 mu m in diameter) and its virulence for Balb/c mice increased about 1000-f old. We conclude that the virulence of the attenuated vaccine strain, F. tularensis LVS, may still be influenced by growth conditions.