IN-VITRO SENSITIVITY OF MEDICALLY SIGNIFICANT FUSARIUM SPECIES TO VARIOUS ANTIMYCOTICS

Sixteen isolates belonging to Fusarium chlamydosporum (n = 4), Fusariu m equiseti (n = 1), Fusarium moniliforme (n = 2), Fusarium oxysporum ( n = 3), Fusarium proliferatum (n = 1), and Fusarium solani (n = 5) wer e tested against amphotericin B, 5-fluorocytosine, fluconazole, itraco nazole, ketoconazole, JAI-amphotericin B (water-soluble compound), ham ycin and amphotericin B combined with 5-fluorocytosine, using antibiot ic medium M3, high-resolution broth (pH 7.1), Sabouraud's dextrose, an d yeast-nitrogen broth media (1 ml/tube). The minimal inhibitory and m inimal fungicidal concentrations of 5-fluorocytosine and fluconazole f or all species were > 100 mu g/ml. All Fusarium isolates, except F. eq uiseti (3.125 mu g), gave minimal inhibitory concentrations of 12.5-10 0 mu g/ml for hamycin. The values for amphotericin B, itraconazole, ke toconazole, JAI-amphotericin B, and amphotericin B combined with 5-flu orocytosine were 1.56-100, 0.78-50, 3.125-100, 50-100, and 1.56 to > 1 00 mu g/ml, respectively. Although a wide range of minimal inhibitory concentrations was recorded for most of the isolates studied, it appea rs that some - F. solani, F. oxysporum, F. chlamydosporum, F. equiseti , and F. moliniforme - were more susceptible to amphotericin B, itraco nazole, ketoconazole, hamycin, and amphotericin B in the presence of 5 -fluorocytosine. All isolates showed resistance to 5-fluorocytosine an d fluconazole. The minimal fungicidal concentrations were either the s ame or several times higher than the minimal inhibitory concentrations .