THE BOVINE PAPILLOMAVIRUS TYPE-1 E5 TRANSFORMING PROTEIN SPECIFICALLYBINDS AND ACTIVATES THE BETA-TYPE RECEPTOR FOR THE PLATELET-DERIVED GROWTH-FACTOR BUT NOT OTHER RELATED TYROSINE KINASE-CONTAINING RECEPTORS TO INDUCE CELLULAR-TRANSFORMATION
Dj. Goldstein et al., THE BOVINE PAPILLOMAVIRUS TYPE-1 E5 TRANSFORMING PROTEIN SPECIFICALLYBINDS AND ACTIVATES THE BETA-TYPE RECEPTOR FOR THE PLATELET-DERIVED GROWTH-FACTOR BUT NOT OTHER RELATED TYROSINE KINASE-CONTAINING RECEPTORS TO INDUCE CELLULAR-TRANSFORMATION, Journal of virology, 68(7), 1994, pp. 4432-4441
The 44-amino-acid E5 protein of bovine papillomavirus type 1 is a high
ly hydrophobic protein which appears to transform cells through the ac
tivation of growth factor receptors. To investigate the specificity of
E5-growth factor receptor interactions required for mitogenic signali
ng, we utilized a nontumorigenic, murine myeloid cell line (32D) which
is strictly dependent on interleukin-3 (IL-3) for sustained prolifera
tion in culture. This IL-3 dependence can be functionally substituted
by the expression of a variety of surrogate growth factor receptors an
d the addition of the corresponding ligand. Several receptor cDNAs for
the alpha- and beta-type platelet-derived growth factor receptors [al
pha PDGFR and beta PDGFR], the epidermal growth factor receptor, and t
he colony-stimulating factor 1 receptor) were transfected into 32D cel
ls constitutively expressing the E5 protein to test for IL-3-independe
nt growth. Only beta PDGFR war capable of abrogating the IL-3 dependen
ce of 32D cells. The proliferative signal induced by the coexpression
of beta PDGFR and E5 was accompanied by stable complex formation betwe
en these proteins, constitutive tyrosine phosphorylation of the recept
or, and tumorigenicity in nude mice. The lack of cooperative interacti
on between E5 and the epidermal growth factor receptor, the colony-sti
mulating factor 1 receptor, and the highly related a PDGFR was paralle
led by the inability of E5 to bind to these receptors and failure to i
ncrease receptor tyrosine phosphorylation. Thus, these data indicate t
hat the ability of ES to induce sustained proliferation and transforma
tion of 32D cells is a direct consequence of specific interaction betw
een the E5 protein and the beta PDGFR signaling complex and the subseq
uent stimulation of receptor tyrosine phosphorylation.