Kj. Siranosian et Ad. Grossman, ACTIVATION OF SPO0A TRANSCRIPTION BY SIGMA(H) IS NECESSARY FOR SPORULATION BUT NOT FOR COMPETENCE IN BACILLUS-SUBTILIS, Journal of bacteriology, 176(12), 1994, pp. 3812-3815
spo0A and spo0H are needed for the initiation of sporulation and for t
he development of genetic competence in Bacillus subtilis. Transcripti
on of spo0A initiates from two promoters, Pv and Ps. Pv is active duri
ng vegetative growth and is recognized by RNA polymerase containing si
gma(A). Expression from Ps increases during sporulation and depends on
sigma(H), the spo0H gene product. A deletion mutation, spo0A Delta Ps
, that removes the promoter controlled by sigma H blocked sporulation
but had no detectable effect on competence. These results indicate tha
t expression of spo0A from Ps is necessary for sporulation and that th
e requirement for spo0H in competence development is not due to its ro
le in expression of spo0A.