THE RULES OF BACTERIAL LUMINESCENCE INHIB ITION IN-VITRO BY CHINONES AND PHENOLS - COMPONENTS OF SEWAGE

The extinguishing of bioluminescence of the bi-fermental system: bacte rial lucipherase-NADH:PMN-oxireductase is considered from the point of view of the rules of the inter-molecular electron and proton transfer between the donor (bioluminescense center) and the acceptor (the anal yzed compound). The relation of a bioluminescense induction period to an oxidative-reductive potential of chinone acceptor is confirmed. It has been shown, that the induction period and the time, when biolumine scense becomes maximal in the presence of chinones are determined by a size of aromatic and aliphatic fragments of chinone molecule; a biolu minescense intensity depends on a efficiency of processes of inter-mol ecular electron and proton transfer (donor-acceptor) and on a concurre nce of the analyzed compound with the aldehyde for the binding site to lucipherase.