DETECTION OF BCRABL FUSION MESSENGER-RNA IN SAMPLES OF WHOLE, UNFRACTIONATED BLOOD

Authors
KOLBE T CUDDY K BRODSKY I MARKS DI GILLESPIE D
Citation
T. Kolbe et al., DETECTION OF BCRABL FUSION MESSENGER-RNA IN SAMPLES OF WHOLE, UNFRACTIONATED BLOOD, Leukemia research, 18(6), 1994, pp. 465-468
Citations number
4
Categorie Soggetti
Oncology,Hematology
Journal title
Leukemia research → ACNP
ISSN journal
01452126
Volume
18
Issue
6
Year of publication
1994
Pages
465 - 468
Database
ISI
SICI code
0145-2126(1994)18:6<465:DOBFMI>2.0.ZU;2-A
Abstract
Using a new strategy, capture-RT-PCR, bcrabl fusion mRNA sequences wer e specifically and sensitively detected in samples of whole blood from leukemia patients with the Philadelphia chromosome. Sample processing required only mixing blood with the chaotropic salt, GuSCN, and mRNA was captured during a short incubation of prepared blood with an affin ity membrane. Immobilized mRNA sequences were amplified without elutio n. No radioisotopes or Southern transfer were needed.