Rjc. Slebos et al., P53-DEPENDENT G(1) ARREST INVOLVES PRB-RELATED PROTEINS AND IS DISRUPTED BY THE HUMAN PAPILLOMAVIRUS-16-E7 ONCOPROTEIN, Proceedings of the National Academy of Sciences of the United Statesof America, 91(12), 1994, pp. 5320-5324
The cell cycle regulatory tumor suppressor proteins p53 and pRB are ta
rgeted for inactivation by several tumor viruses, including the high-r
isk types of human papillomaviruses (HPVs) via interactions of the HPV
E6 and E7 oncoproteins with p53 and pRB, respectively. p53 plays a ce
ntral role in a signal transduction pathway that mediates G(1) arrest
after DNA damage, though the mechanism by which G(1) arrest occurs has
not been elucidated, The cyclin-associated protein p21(waf1/cip1) has
recently been shown to be induced by p53 and to inhibit cyclin comple
x-mediated phosphorylation of pRB in vitro. Thus, we investigated a po
ssible role for pRB in the p53-mediated DNA damage response. After gam
ma-irradiation, cells expressing wild-type p53 arrested in G(1), conta
ined increased levels of WAF1/CIP1 mRNA, and demonstrated accumulation
of hypophosphorylated pRB. In contrast, cell lines with abnormal p53
genes or with p53 functionally inactivated by the E6 oncoprotein of HP
V16 (a high-risk HPV) failed to arrest in G(1), did not elevate WAF1/C
IP1 mRNA, and did not accumulate hypophosphorylated pRB. Despite appar
ently normal elevation of p53 protein and WAF1/CIP1 mRNA after irradia
tion, cells expressing HPV16 E7 also faded to arrest in G(1) and did n
ot accumulate hypophosphorylated pRB. Disruption of RB genes alone did
not totally abrogate this G(1) arrest. Our results suggest that p53 i
ndirectly regulates phosphorylation of pRB and that pRB and/or other p
RB-like molecules that bind to HPV16 E7 participate in the DNA damage-
mediated G(1) arrest signal. In the process of HPV infection, the HPV
E6 and E7 oncoproteins may undermine this cell cycle checkpoint, contr
ibuting to the accumulation of genetic alterations during tumorigenesi
s.