MUTAGEN FORMATION AFTER HEATING OF MIXTURES OF GUANIDINO COMPOUNDS, AMINO-ACIDS AND GLUCOSE

The effect of guanidino compounds other than creatin(in)e on mutagen f ormation was tested after heating with amino acid and glucose in a sol ution at 128-degrees-C for 2 h. The mutagenicity of a heated mixture o f creatine, glycine and glucose to Salmonella typhimurium TA98 with S9 mix was 630 per mg of heated material. When L-arginine was mixed with glycine plus glucose, 39 revertants of TA98 were induced per mg of he ated material. Other guanidino compounds such as N(G)-methyl-L-arginin e, 1-methylguanidine, aminoguanidine and guanidine also yielded mutage ns, inducing 6.0-64 revertants per mg of heated material. When guanidi no compounds were heated with L-phenylalanine and glucose, mixtures al so yielded mutagenicity. The mutagenicity of a heated mixture of creat ine, L-phenylalanine and glucose to TA98 with S9 mix was 160 per mg of heated material. L-Arginine, N(G)-methyl-L-arginine, aminoguanidine a nd guanidine gave 1.9 approximately 21 revertants per mg of heated mat erial. With the addition of 1-methylguanidine, a very potent mutagenic ity of 200 per mg of heated material was observed.