U. Gerike et P. Dimroth, EXPRESSION OF SUBUNIT-A AND SUBUNIT-C OF THE SODIUM-DEPENDENT ATPASE OF PROPIONIGENIUM-MODESTUM IN ESCHERICHIA-COLI, Archives of microbiology, 161(6), 1994, pp. 495-500
The aim of the present study was to construct functional hybrid ATPase
s consisting of all Escherichia coli ATPase subunits excepts the F-0 s
ubunits a or c which were replaced by the respective subunits of the P
ropionigenium modestum ATPase. This would give valuable information on
the subunit(s) conferring the coupling ion specificity. Plasmids were
constructed that carried the gene for subunit c (uncE) or subunit a (
uncB) behind a tac promoter. These plasmids were transformed into E. c
oli strains which differed with respect to the une operon and the expr
ession of the P. modestum genes was verified biochemically. Enhanced e
xpression of the P. modestum genes led to strong growth inhibition of
all E. coli strains tested. However, the expressed P. modestum protein
s could not functionally complement E. coli strains that lacked the ho
mologous subunit.