ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY AND NEUTRALIZATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 BY HIGH-AFFINITY CROSS-LINKING OF GP41 TO HUMAN MACROPHAGE FC IGG RECEPTOR USING BISPECIFIC ANTIBODY

Human monocytes/macrophages, which express Fc receptors for IgG are in volved in human immuno-deficiency virus type 1 (HIV-1) infection and p athogenesis. These receptors are known to mediate numerous immunologic al functions including cell-mediated killing and possible targeting of HIV to the lysophagosome monocyte-derived macrophage (MDM) entry rout e for virus neutralization. To study both activities in HIV-1 infectio n, MDM Fc gamma RI was specifically selected using bispecific antibody (Bs-Ab) containing whole human monoclonal antibody against gp41 and t he Fab' fragment of murine anti-Fc gamma RI 22.2 antibody. Bs-Ab was f ound to mediate potent antibody-dependent cellular cytotoxicity and vi rus neutralization.