Pk. Inamdar et Rd. Yeole, DETERMINATION OF FLAVOPIRIDOL (L-86-8275) IN RAT SERUM USING ION-PAIRREVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Drug development and industrial pharmacy, 23(3), 1997, pp. 305-308
A specific, sensitive, and simple high-performance liquid chromatograp
hic method has been developed for assaying flavopiridol in rat serum f
or preliminary bioavailability and pharmacokinetic studies. The drug a
nd internal standard were extracted with dichloromethane from serum. A
fter evaporating the solvent, the extract was reconstituted with mobil
e phase. Chromatography was performed on a 10-mu Bondapak C-18 column
(30 cm x 3.9 mm ID), using methanol:water (50:50, v/v) containing 0.05
M ion-pairing reagent PIC B-6 as a mobile phase at a flow rate of 1.1
ml/min, with ultraviolet detection at 278 nm. The drug and internal s
tandard were well resolved from other serum constituents. Due to high
sensitivity and reproducibility the method can be adapted very well fo
r assaying flavopiridol in higher animals.