DETERMINATION OF FLAVOPIRIDOL (L-86-8275) IN RAT SERUM USING ION-PAIRREVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A specific, sensitive, and simple high-performance liquid chromatograp hic method has been developed for assaying flavopiridol in rat serum f or preliminary bioavailability and pharmacokinetic studies. The drug a nd internal standard were extracted with dichloromethane from serum. A fter evaporating the solvent, the extract was reconstituted with mobil e phase. Chromatography was performed on a 10-mu Bondapak C-18 column (30 cm x 3.9 mm ID), using methanol:water (50:50, v/v) containing 0.05 M ion-pairing reagent PIC B-6 as a mobile phase at a flow rate of 1.1 ml/min, with ultraviolet detection at 278 nm. The drug and internal s tandard were well resolved from other serum constituents. Due to high sensitivity and reproducibility the method can be adapted very well fo r assaying flavopiridol in higher animals.