SENSITIVITY OF THE PLATELET IMMUNOFLUORESCENCE TEST (PIFT) AND THE MAIPA ASSAY FOR THE DETECTION OF PLATELET-REACTIVE ALLOANTIBODIES - A REPORT ON 2 UK NATIONAL PLATELET WORKSHOP EXERCISES

Authors
ALLEN DL CHAPMAN J PHILLIPS PK OUWEHAND WH
Citation
Dl. Allen et al., SENSITIVITY OF THE PLATELET IMMUNOFLUORESCENCE TEST (PIFT) AND THE MAIPA ASSAY FOR THE DETECTION OF PLATELET-REACTIVE ALLOANTIBODIES - A REPORT ON 2 UK NATIONAL PLATELET WORKSHOP EXERCISES, TRANSFUSION MEDICINE, 4(2), 1994, pp. 157-164
Citations number
14
Categorie Soggetti
Hematology
Journal title
TRANSFUSION MEDICINE → ACNP
ISSN journal
09587578
Volume
4
Issue
2
Year of publication
1994
Pages
157 - 164
Database
ISI
SICI code
0958-7578(1994)4:2<157:SOTPIT>2.0.ZU;2-8
Abstract
This report presents the results of two National Workshop exercises wh ich were designed to evaluate interlaboratory and interassay variation in sensitivity of techniques used for the detection of platelet-react ive alloantibodies. Most workshop participants used the platelet immun ofluorescence test. Sensitivity of this assay was improved when fluore scence was measured by flow cytometer rather than by microscope. The M AIPA assay was found to be highly sensitive but required considerable technical expertise, and the choice of antiglycoprotein IIb/IIIa (CD61 /41) monoclonal antibody was found to have a significant effect on its ability to detect anti-HPA-1a.