EFFECTS OF CALCIUM, ALGINATE, AND CALCIUM-ALGINATE IMMOBILIZATION ON GROWTH AND TROPANE ALKALOID LEVELS OF A STABLE SUSPENSION CELL-LINE OFDATURA-INNOXIA MILL

Authors
GONTIER E SANGWAN BS BARBOTIN JN
Citation
E. Gontier et al., EFFECTS OF CALCIUM, ALGINATE, AND CALCIUM-ALGINATE IMMOBILIZATION ON GROWTH AND TROPANE ALKALOID LEVELS OF A STABLE SUSPENSION CELL-LINE OFDATURA-INNOXIA MILL, Plant cell reports, 13(9), 1994, pp. 533-536
Citations number
23
Categorie Soggetti
Plant Sciences
Journal title
Plant cell reports → ACNP
ISSN journal
07217714
Volume
13
Issue
9
Year of publication
1994
Pages
533 - 536
Database
ISI
SICI code
0721-7714(1994)13:9<533:EOCAAC>2.0.ZU;2-8
Abstract
A stabilized two-year old suspension of a Datura innoxia cell line, pr oducing small amounts of tropane alkaloids (scopolamine and hyoscyamin e) was used in this study. Calcium alginate immobilization has been sh own to be able to increase secondary metabolite (i, e. alkaloid) produ ction. The effects of calcium and ungellified alginate were both benef icial for tropane alkaloid synthesis; a 10mM calcium chloride supply g ave the best results, with a 10-fold yield increase.