ANGIOTENSIN-II RECEPTORS IN THE HUMAN ADRENAL-GLAND

In the present study, we have characterized distribution and pharmacol ogical properties of angiotensin II (Ang II) receptors in human adrena ls frozen immediately after removal. Autoradiographic studies indicate that Ang II receptors are present throughout the gland. Co-incubation s with DUP 753, a specific antagonist of the AT(1) receptor, and with PD 123319, a specific antagonist of the AT(2) receptor, reveal that An g II receptors are mainly of type 2. The AT(1) receptors are detected after 16 weeks of gestation at the periphery of the gland. Competition studies and Scatchard analysis reveal a homogenous population of high affinity AT(2) binding sites (Kd = 0.68 +/- 0.1 nM). Binding capaciti es decrease from 1080 +/- 304 fmol/mg protein at 14 weeks to 275 +/- 5 5 fmol/mg protein at 21 weeks. These results differ from those obtaine d in adult glands where autoradiographic studies reveal that the AT(1) receptors are found mainly in the zona glomerulosa and AT(2) receptor s mainly in the medulla. These data suggest that the AT(2) receptors c ould be involved in the morphological or functional differentiation of the human fetal adrenal gland.