NONRANDOM CHANGES OF CHROMOSOME-10 IN BLADDER-CANCER - DETECTION BY FISH TO INTERPHASE NUCLEI

Fluorescence in situ hybridization (FISH) to interphase nuclei has bee n a valuable method for examining the chromosome copies in tumor cells in clinical practice. Twelve cases of transitional cell carcinoma (TC C) of the bladder were investigated with a biotin-labeled repetitive D NA probe to detect numerical aberrations of chromosome 10 in interphas e nuclei. The cells containing one fluorescent signal were screened in two of seven non-invasive tumors and in four of five invasive tumors. Two patients presented two FISH spots of different sizes. More than t wo signals were seen in one invasive tumor. The findings suggest that partial or complete loss of a chromosome 10 is a nonrandom aberration in bladder cancer.