HETEROLOGOUS GENE-EXPRESSION AND PROTEIN SECRETION FROM CANDIDA-GLABRATA

We have examined heterologous protein secretion from Candida glabrata with the aid of a stable C. glabrata vector and a secretion reporter c assette comprising the Saccharomyces cerevisiae PGK-gene promoter and a Kluveromyces lactis secretion signal to drive secretion of Escherich ia coli beta-lactamase. Abundant secretion of beta-lactamase from C. g labrata indicates that the S. cerevisiae PGK promoter functions in C. glabrata. Furthermore, we show that C. glabrata processes the secreted beta-lactamase in a manner similar to, but not identical with, S. cer evisiae and K. lactis. C. glabrata may be a suitable new host for the expression of foreign genes.