K. Ohki et O. Kohashi, LAMININ PROMOTES PROLIFERATION OF BONE-MARROW-DERIVED MACROPHAGES ANDMACROPHAGE CELL-LINES, Cell structure and function, 19(2), 1994, pp. 63-71
We examined the effect of laminin on DNA synthesis or cell growth of b
one marrow-derived macrophages (BMM) and five macrophage cell lines in
cluding two factor-dependent macrophage cell lines (BDM-1 and BDM-1W3)
and three factor-independent ones (BAM3, J774.1, and P388D1). Laminin
stimulated the M-CSF-dependent proliferation of BMM and BDM-1 cells i
n a dose-dependent manner, but it had no effect on the proliferation o
f BDM-1W3 cells. This stimulatory effect was modified by increasing th
e concentration of fetal calf serum in the culture medium. Laminin als
o stimulated the proliferation of BAM3 and J774.1 cells, whereas it co
uld not stimulate that of P388D1 cells. Taken together, these results
suggest that the differences in the response of these macrophage cell
lines to the presence of laminin may be derived from the difference in
the state of maturation of these cells. Although laminin acted as a p
otent mitogenic factor when it was added to the culture medium, the st
imulation was two- to threefold lower when dishes were coated with equ
ivalent amounts of laminin. When BDM-1 cells were cultured with lamini
n, they underwent drastic morphological changes. A monoclonal antibody
that recognizes the integrin as subunit only partially inhibited the
mitogenic effect of laminin. The results in this report suggest that l
aminin may participate in the regulation of macrophage populations in
vivo.