GENERALIZED AND MOBILIZABLE POSITIVE-SELECTION CLONING VECTORS

We have modified the positive-selection cloning vector pUN121 to expan d the numbers of unique cloning sites by insertion of a multiple cloni ng site into the h cl gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzy me sites suitable for general cloning purposes. A mobilizable version (pSKM11) of pSKM10 was constructed by insertion of the IncP mob sequen ce which permitted mobilization of the plasmid into a wide variety of Gram(-) bacteria.