M. Bambagiottialberti et al., CHARACTERIZATION OF HEMATOLOGY LYSING REAGENTS CONTAINING QUATERNARY AMMONIUM HALIDES USING FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 8(6), 1994, pp. 439-442
Positive- and negative-ion fast-atom bombardment (FAB) mass spectromet
ry has been employed to characterize pure dodecyl-, tetradecyl- and he
xadecyl-trimethylammonium chlorides and bromides, compounds of high in
terest as lysing agents in hematologic analyses. In parallel, the same
approach has been used for some commercially available lysing reagent
products. While in positive-ion FAB mass spectra the presence of the
abundant ions corresponding to the intact quaternary ammonium cations
(Q+) is accompanied by the presence of [Q2X]+ (X = Cl, Br) species, in
the negative-ion mode cluster ions such as [QX2]- are the sole ionic
species that are easily evident. The mechanism for the formation of [Q
2X]+ and [QX2]- species has been studied and some suggested explanatio
ns are given.