SEPARATION OF AMYLOGLUCOSIDASE USING BETA-CYCLODEXTRIN CHITOSAN

The adsorption characteristics of commercial amyloglucosidase enzyme p reparation from Aspergillus niger on the beta-cyclodextrin-chitosan sy stem were studied. The effects of pH, temperature, and ionic strength on the equilibrium partition coefficients were investigated in order t o obtain the optimum process conditions for a possible AMG purificatio n scheme. It was shown that the adsorption isotherm for this system at pH 4.5 at ambient temperature obeyed the Langmuir relationship, and t he Langmuir parameters of q(m) and K(p) were estimated as 120.5 mg/mL hydrated gel and 1.4 mg/mL solvent, respectively. The complete elution of the enzyme from the matrix was achieved with an increase in pH by using 0.1 M borate buffer, pH 8.0.