A PHYSICAL MAP OF 2 CLUSTERS CONTAINING THE GENES FOR 6 PROINFLAMMATORY RECEPTORS

The genes encoding for six receptors involved in the proinflammatory r esponse lie on different chromosomes. Two receptors for N-formylpeptid es (FPR1, FPR2), one homologue of these (FPRL2), and the receptor for complement fragment C5a (C5aR) are encoded by four genes mapped to hum an chromosome 19. The genes encoding two receptors for Interleukin-8 ( IL8RA, IL8RB) have been located on human chromosome 2. In this report we describe the physical linkage between these genes in two different clusters. DNA fragments obtained by digestion with several restriction enzymes were separated by pulsed field gel electrophoresis. Nylon fil ters were hybridized with probes corresponding to the complete transla ted sequences of these genes. These probes were obtained from a human neutrophil cDNA-library. The four genes on chromosome 19 are contained in a 200 kilobase (kb) fragment. Both Interleukin-8 receptors are on a 150 kb fragment. The complete translated sequences for these genes w ere amplified from genomic DNA, indicating that they are contained in a single exon.