Jh. Song et al., DETECTION OF THE H1-J STRAIN OF SALMONELLA-TYPHI AMONG KOREAN ISOLATES BY THE POLYMERASE CHAIN-REACTION, The American journal of tropical medicine and hygiene, 50(5), 1994, pp. 608-611
Citations number
15
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
Salmonella typhi, the etiologic agent of typhoid fever, typically has
only a phase-1 flagellar antigen, H1-d (fliC). While most strains of S
. typhi have H1-d antigen, 10-20% of Indonesian isolates have been rep
orted to possess H1-j antigen instead. To investigate the presence H1-
j strains of S. typhi isolates in Korea, where typhoid fever is still
a common infectious problem, we used the polymerase chain reaction (PC
R) with a pair of oligonucleotides primers that specifically amplified
the flagellin gene of S. typhi. Of 375 isolates of S. typhi tested, o
nly one was shown to possess the H1-j antigen, which was shown by the
presence of a 1,269-basepair fragment on agarose gel electrophoresis a
fter the PCR. The isolate with the H1-j antigen was cultured from a Ko
rean-Indonesian man who was already symptomatic in Indonesia and was t
hought to be an Indonesian strain. Because 375 strains tested in this
study were collected from cases with typhoid fever in different region
s of Korea during the period from 1986 to 1991, it could be concluded
that the mutation rate to j antigen is negligible among S. typhi endem
ic in Korea.