DETECTION OF PORCINE REPRODUCTIVE RESPIRATORY SYNDROME VIRUS BY THE POLYMERASE CHAIN-REACTION

Authors
VANWOENSEL P VANDERWOUW J VISSER N
Citation
P. Vanwoensel et al., DETECTION OF PORCINE REPRODUCTIVE RESPIRATORY SYNDROME VIRUS BY THE POLYMERASE CHAIN-REACTION, Journal of virological methods, 47(3), 1994, pp. 273-278
Citations number
9
Categorie Soggetti
Virology,"Biochemical Research Methods
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
47
Issue
3
Year of publication
1994
Pages
273 - 278
Database
ISI
SICI code
0166-0934(1994)47:3<273:DOPRRS>2.0.ZU;2-O
Abstract
A polymerase chain reaction (PCR) is described for the detection of th e porcine reproductive respiratory syndrome virus (PRRSV). Using the P CR we were able to detect about 30 infectious units of PRRSV resolved in tissue culture medium or sperm. For the detection of PRRSV in sperm the PCR is 10-times more sensitive than culturing in alveolar macroph ages. For the detection of PRRSV, PCR provides a good alternative to c ell culture methods.