DETERMINATION OF OCTADECADIENOIC ACIDS IN HUMAN SERUM - A CRITICAL-APPRAISAL

Authors
READ G RICHARDSON NR WICKENS DG
Citation
G. Read et al., DETERMINATION OF OCTADECADIENOIC ACIDS IN HUMAN SERUM - A CRITICAL-APPRAISAL, Analyst, 119(3), 1994, pp. 393-396
Citations number
24
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
119
Issue
3
Year of publication
1994
Pages
393 - 396
Database
ISI
SICI code
0003-2654(1994)119:3<393:DOOAIH>2.0.ZU;2-N
Abstract
The method based on high-performance liquid chromatography currently u sed to determine the lipid levels of octadeca-9(Z),11(E)-dienoic acid in human serum fails to distinguish between this acid and octadeca-10( E),12(Z)-dienoic acid. Adducts with the fluorescent dienophile -[3-(1- propenyl)propyl]-1,2,4-triazoline-3,5-dione permit the separation of t hese two isomeric acids as does gas-liquid chromatography of their met hyl esters, but these alternatives produce equivocal results when appl ied to biological samples.