CHARACTERIZATION OF THE HEMODYNAMIC-RESPONSE TO INTRAVENOUS DIASPIRINCROSS-LINKED HEMOGLOBIN SOLUTION IN RATS

Diaspirin crosslinked hemoglobin solution (DCLHb(TM)) has potential fo r clinical use as an oxygen-carrying solution because of its excellent oxygen transport properties and biochemical stability. The present st udy characterizes the effects of intravenous infusions of 0.625-40 mL/ kg (62.5-4000 mg/kg) DCLHb on mean blood pressure (MAP) and heart rate (HR) in conscious rats. DCLHb at all doses tested except 62.5 mg/kg w as associated with an immediate increase in MAP (25-30% above baseline ) that peaked between 20-30 minutes after infusion and returned to bas eline within 120-300 minutes in a dose-dependent manner. Maximum MAP a chieved was in the range of 129 +/- 7 to 140 +/- 7 mm Hg and there was no statistically significant difference in the response between doses . HR responded in a reciprocal manner to changes in MAP. Volume- and o ncotic-matched infusions of LR and albumin did not alter MAP or HR. Sl ow infusion (0.34 mL/min) of DCLHb appeared to blunt the magnitude of the presser response when compared to bolus injection (<10 sec). DCLHb administration is associated with a presser response that is not due to volume load, oncotic pressure, or rate of infusion, suggesting that it is intrinsic to the modified hemoglobin molecule and pharmacologic in nature.