THIN-FILM CONDUCTOMETRIC ENZYME BIOSENSORS FOR THE DETERMINATION OF GLUCOSE AND UREA IN BLOOD

Authors
SHULGA AA DZYADEVICH SV SOLDATKIN AP PATSKOVSKII SV STARODUB NF STRIKHA VI ELSKAYA AV
Citation
Aa. Shulga et al., THIN-FILM CONDUCTOMETRIC ENZYME BIOSENSORS FOR THE DETERMINATION OF GLUCOSE AND UREA IN BLOOD, Russian electrochemistry, 29(8), 1993, pp. 1219-1224
Citations number
10
Categorie Soggetti
Electrochemistry
Journal title
Russian electrochemistry → ACNP
ISSN journal
10703276
Volume
29
Issue
8
Year of publication
1993
Pages
1219 - 1224
Database
ISI
SICI code
1070-3276(1993)29:8<1219:TCEBFT>2.0.ZU;2-C
Abstract
The characteristics of enzyme biosensors built with thin-film Au, Ti, and Al electrodes of different geometry were studied. The highest sens itivity was found to be attained with gold electrodes. Biomembranes co ntaining glucose oxidase or urease as the enzyme immobilized by covale nt crosslinking with bovine serum albumin with the aid of glutaraldehy de were formed on the electrode surfaces in order to create the biosen sors. The sensors obtained had a linear dynamic range from 0.1 to 3.0 mM for urea and from 0.1 to 2.0 mM for glucose; their respective sensi tivities were 50 and 8 muOMEGA-1/mM when measuring in 5 mM potassium p hosphate buffer of pH 7.4. Results for glucose and urea in blood found with these sensors were reported.