STABILIZATION OF TRYPSIN BY GLYCOSYLATION

Authors
VANKOVA H",RP,"POSPISILOVA M TICHA M TURKOVA J
Citation
M. Vankova H",rp,"pospisilova et al., STABILIZATION OF TRYPSIN BY GLYCOSYLATION, Biotechnology techniques, 8(6), 1994, pp. 375-380
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
6
Year of publication
1994
Pages
375 - 380
Database
ISI
SICI code
0951-208X(1994)8:6<375:SOTBG>2.0.ZU;2-T
Abstract
The stabilization of trypsin against thermal inactivation and autolysi s was achieved by coupling saccharides to lysine residues of the enzym e. The reaction of reductive amination was used to bind reducing disac charides. Periodate oxidized saccharide residues of the modified prote in were used for coupling of trypsin to solid supports containing amin o or hydrazide groups.