A SIMPLE STAINING PROCEDURE FOR THE CHARACTERIZATION OF BASIDIOMYCETES PELLETS BY IMAGE-ANALYSIS

Authors
DURANT G CRAWLEY G FORMISYN P
Citation
G. Durant et al., A SIMPLE STAINING PROCEDURE FOR THE CHARACTERIZATION OF BASIDIOMYCETES PELLETS BY IMAGE-ANALYSIS, Biotechnology techniques, 8(6), 1994, pp. 395-400
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
6
Year of publication
1994
Pages
395 - 400
Database
ISI
SICI code
0951-208X(1994)8:6<395:ASSPFT>2.0.ZU;2-N
Abstract
A simple staining procedure, associated with image analysis, was used to discriminate two zones (the filamentous zones and the compacted cor es) in aggregates of fungal biomass according to their accessibility f or an agent of the medium. The application of the method to batch ferm entations of Schizophyllum commune showed a higher proportion of loose ly entangled particles (clumps) and thinner filamentous outer zones ob tained at a high agitation speed (500 rpm) compared to low agitation s peeds (120-200 rpm).