PRODUCTION OF ENDO-1,4-BETA-GLUCANASE AND XYLANASE BY TRICHODERMA-REESEI IMMOBILIZED ON POLYURETHANE FOAM

Endo-1,4-beta-glucanase and xylanase were produced by Trichoderma rees ei immobilized on polyurethane foam using lactose as the main carbon s ource. The most porous carrier was found to be the best of those teste d. The nitrogen source and KH2PO4 concentration of the production medi um had a marked effect on culture pH during the course of fermentation and, consequently, on xylanase activity. An increase in lactose conce ntration from 7 to 27 g/l resulted in an increase in endoglucanase act ivity (max. 730 U/ml), xylanase activity (max. 3350 U/ml) and filter p aper activity (max. 3.0 FPU/ml).