Al. Knight et al., SURVEY OF AZINPHOSMETHYL RESISTANCE IN CODLING MOTH (LEPIDOPTERA, TORTRICIDAE) IN WASHINGTON AND UTAH, Journal of economic entomology, 87(2), 1994, pp. 285-292
A bioassay in which azinphosmethyl was incorporated into the adhesive
of a sex pheromone trap was developed to detect resistance in populati
ons of male codling moth, Cydia pomonella (L.). Experiments were done
to evaluate the influence of the length of the bioassay, moth body pos
ition in the adhesive, moth age, and extremes of temperature and relat
ive humidity (RH) experienced by moths caught in traps in the field. M
oth's body position and age for moths <5 d old were not significant fa
ctors affecting moth mortality. Exposure to low humidity (45% RH) or l
ow to high temperature (10-20-degrees-C) for up to 12 h did not signif
icantly affect mortality. Forty-eight populations were surveyed in Was
hington, and three populations were surveyed in Utah during 1991-1992
with a discriminating concentration, 75 mug (AI)/g adhesive; moth mort
ality was scored after 64 h at 20-degrees-C and >85% RH. Populations f
rom crabapple plantings, certified-organic, abandoned, and conventiona
l orchards were sampled. Twenty of 44 populations monitored in 1991 an
d 12 of 17 populations monitored in 1992 had significant levels of res
istance compared with a susceptible laboratory strain based on nonover
lapping 95% CL. All but one of these resistant populations were from c
urrent conventional or recently abandoned conventional orchards. Resul
ts from the 10 orchards sampled in both years were not significantly d
ifferent. Resistance in a population collected in 1991 reverted rapidl
y when removed from exposure to azinphosmethyl and reared on apple in
the laboratory.