Mg. Cecchini et al., ROLE OF COLONY-STIMULATING FACTOR-I IN THE ESTABLISHMENT AND REGULATION OF TISSUE MACROPHAGES DURING POSTNATAL-DEVELOPMENT OF THE MOUSE, Development, 120(6), 1994, pp. 1357-1372
Colony stimulating factor-1 (CSF-1) regulates the survival, proliferat
ion and differentiation of mononuclear phagocytes. The osteopetrotic (
op/op) mutant mouse is devoid of CSF-1 due to an inactivating mutation
in the CSF-1 gene and is deficient in several mononuclear phagocyte s
ubpopulations. To analyze more fully the requirement for CSF-1 in the
establishment and maintenance of mononuclear phagocytes, the postnatal
development of cells bearing the macrophage marker antigens F4/80 and
MOMA-1, in op/op mice and their normal (+/op or +/+) littermates, wer
e studied during the first three months of life. In normal mice, maxim
um expression of tissue F4/80+ cells was generally correlated with the
period of maximum organogenesis and/or cell turnover. Depending on th
e tissue, the F4/80+ cell density either decreased, transiently increa
sed or gradually increased with age. In op/op mice, tissues that norma
lly contain F4/80+ cells could be classified into those in which F4/80
+ cells were absent and those in which the F4/80+ cell densities were
either reduced, normal or initially normal then subsequently reduced.
To assess which F4/80+ populations were regulated by circulating CSF-1
in normal mice, op/op mice in which the circulating CSF-1 concentrati
on was restored to above normal levels by daily subcutaneous injection
of human recombinant CSF-1 from day 3 were analyzed. These studies su
ggest that circulating CSF-1 exclusively regulates both the F4/80+ cel
ls in the liver, spleen and kidney and the MOMA-1+ metallophilic macro
phages in the spleen. Macrophages of the dermis, bladder, bone marrow
and salivary gland, together with a subpopulation in the gut, were par
tially restored by circulating CSF-1, whereas macrophages of the muscl
e, tendon, periosteum, synovial membrane, adrenals and the macrophages
intimately associated with the epithelia of the digestive tract, were
not corrected by restoration of circulating CSF-1, suggesting that th
ey are exclusively locally regulated by this growth factor. Langerhans
cells, bone marrow monocytes and macrophages of the thymus and lymph
nodes were not significantly affected by circulating CSF-1 nor decreas
ed in op/op mice, consistent with their regulation by other growth fac
tors. These results indicate that important differences exist among mo
nonuclear phagocytes in their dependency on CSF-1 and the way in which
CSF-1 is presented to them. They also suggest that the prevalent role
of CSF-1 is to influence organogenesis and tissue turnover by stimula
ting the production of tissue macrophages with local trophic and/or sc
avenger (physiological) functions. Macrophages involved in inflammator
y and immune (pathological) responses appear to be dependent on other
factors for their ontogenesis and function. This study provides a base
from which to analyze further the mechanisms of regulation and physio
logical roles of CSF-l-dependent tissue macrophages.