RAPID DIAGNOSIS OF TUBERCULOUS MENINGITIS BY A SIMPLIFIED NESTED AMPLIFICATION PROTOCOL

Authors
LIU PYF SHI ZY LAU YJ HU BS
Citation
Pyf. Liu et al., RAPID DIAGNOSIS OF TUBERCULOUS MENINGITIS BY A SIMPLIFIED NESTED AMPLIFICATION PROTOCOL, Neurology, 44(6), 1994, pp. 1161-1164
Citations number
10
Categorie Soggetti
Clinical Neurology
Journal title
Neurology → ACNP
ISSN journal
00283878
Volume
44
Issue
6
Year of publication
1994
Pages
1161 - 1164
Database
ISI
SICI code
0028-3878(1994)44:6<1161:RDOTMB>2.0.ZU;2-T
Abstract
We have developed a simplified protocol based on nested polymerase cha in reaction (PCR) for early diagnosis of tuberculous meningitis. Using this protocol, we detected the Mycobacterium tuberculosis genome with in 24 hours in the CSF of 19 of 21 patients (90%) with clinically susp ected tuberculous meningitis. The PCR results were negative in all 79 nontuberculous meningitis controls.