PRODUCTION OF HIGH QUANTITIES OF 3-ACETYLDEOXYNIVALENOL AND DEOXYNIVALENOL

The time course of 3-acetyldeoxynivalenol (AcDON) formation was analys ed over a 11 day culture period of Fusarium graminearum (DSMO 4258) on rice. The maximum of 2840 mg/kg AcDON was detected after 9 days of cu lture. To overcome the complicated clean up of the solid substrate ext ract, 10% methanol in water was applied as extract solvent. After liqu id-liquid partition with ethyl acetate, more than 75% of the toxin cou ld be detected. After one simple clean up step (column chromatography over florisil) AcDON could be crystallised. After ion exchange chromat ography deoxynivalenol (DON) could be crystallised and an overall yiel d of 1,44 g DON per kg of culture was obtained.