GABA AND GLUTAMATE IN MICROVESICLES OF NEUROHYPOPHYSIS - IMMUNOCYTOCHEMICAL STUDY

By using immunogold electron microscopy technique we have demonstrated the presence of GABA and glutamate in the microvesicles of rat neuroh ypophysis. The distribution of neurotransmitters was revealed using mo noclonal antibody against glutamate and polyclonal antibody against GA BA with colloidal gold labelling. Distribution of gold particles was s imilar for both neurotransmitters being maximal over heterogenous popu lations of microvesicles and mitochondria. After 10 min cerebral ische mia immunolabelling of microvesicles was more intense, especially for glutamate. The role of glutamate toxicity after ischemia is discussed.