The variability of the cocoa (Theobroma cacao) nuclear genome was inve
stigated. A total of 203 cocoa clones was surveyed for restriction fra
gment length polymorphisms (RFLPs) using four restriction endonuclease
and 31 seed cDNA probes. A high level of polymorphism has been found.
This study points to a structuring of the species that fits with the
distinction between the Criollo and Forastero populations. These resul
ts combined with previously obtained nuclear rDNA and mtDNA data allow
us to propose new hypotheses on the origin and evolution of the diffe
rent cocoa populations.