We describe six new mutants, spontaneous or EMS-induced, and a previou
sly isolated radiation-induced mutant of Megaselia scalaris Loew (Dipt
era, Phoridae). Five are eye-color mutants, one affects the segmentati
on of the abdomen, and one disturbs the regular form and arrangement o
f ommatidia in the compound eye. A complementation test exposed two pa
irs of alleles among the five eye-color mutants, leaving three differe
nt loci that affect eye pigmentation. Two of them influence the amount
of xanthommatin synthesized, and one blocks the ommochrome pathway at
the kynurenine step. We established chromosome assignment of the muta
nts by crossbreeding them with Y chromosome-autosome translocation str
ains.