T. Dharakul et al., DENGUE VIRUS-SPECIFIC MEMORY T-CELL RESPONSES IN HUMAN VOLUNTEERS RECEIVING A LIVE ATTENUATED DENGUE VIRUS TYPE-2 CANDIDATE VACCINE, The Journal of infectious diseases, 170(1), 1994, pp. 27-33
A live attenuated dengue virus type 2 candidate vaccine (16681-PDK53)
was evaluated in a phase I trial in 10 nonimmune adult volunteers. The
dengue virus-specific memory T cell responses were analyzed as part o
f this study. Dengue virus-specific T cell proliferative responses wer
e observed in all subjects after stimulating their peripheral blood mo
nonuclear cells with live viruses or noninfectious viral antigens. The
highest proliferative response was against dengue virus type 2, altho
ugh cross-reactivity with other flaviviruses was detected to a lesser
degree in some subjects. Dengue virus type 2-specific CD4(+) and CD8() cytotoxic T lymphocytes were generated in all vaccinees. This study
investigated whether the candidate vaccine was efficacious in inducing
dengue virus-specific CD4(+) and CD8(+) T cell memory after a single
immunization in nonimmune recipients.