TYPING OF THE GRANULOCYTE-SPECIFIC NA ANTIGENS BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS

An RNA-based method has been developed to genotype donors for the gran ulocyte-specific alloantigens NA1 and NA2. mRNA was isolated from gran ulocytes, reversely transcribed into cDNA and amplified using an Fc-ga mma-receptor III-1 sequence-specific primer in the polymerase chain re action (PCR). PCR products were analysed by restriction fragment lengt h polymorphism (RFLP) using the restriction endonuclease Tag I, which provided a distinct restriction fragment pattern corresponding to the NA alleles. 17 donors were typed by PCR-RFLP and the results were in c lose accordance with those obtained by serological phenotyping by gran ulocyte immunofluorescence and the antigen capture assay MAIGA.