USE OF ACRIDINIUM-ESTER-LABELED DNA PROBES FOR IDENTIFICATION OF MYCOBACTERIA IN BACTEC 13A BLOOD CULTURES

Authors
EPHRAIM DA SPITZER ED
Citation
Da. Ephraim et Ed. Spitzer, USE OF ACRIDINIUM-ESTER-LABELED DNA PROBES FOR IDENTIFICATION OF MYCOBACTERIA IN BACTEC 13A BLOOD CULTURES, Diagnostic microbiology and infectious disease, 18(3), 1994, pp. 137-139
Citations number
9
Categorie Soggetti
Microbiology,"Infectious Diseases
Journal title
Diagnostic microbiology and infectious disease → ACNP
ISSN journal
07328893
Volume
18
Issue
3
Year of publication
1994
Pages
137 - 139
Database
ISI
SICI code
0732-8893(1994)18:3<137:UOADPF>2.0.ZU;2-5
Abstract
AccuProbe rests for mycobacteria (Gen-Probe) cannot be performed direc tly on BACTEC 13A cultures because of interfering substances. This pro blem can be circumvented by subculturing to BACTEC 12B media. Artifact ual chemiluminescence greater than the positive cutoff was seen in 15 of 19 13A cultures containing Mycobacterium avium complex compared wit h 0 of 19 in 12B subcultures. Of the subcultures, 89% were tested afte r overnight incubation.