H. Ukeda et al., FLOW-INJECTION ANALYSIS OF GLYCEROL BASED ON OXYGEN-CONSUMPTION USINGGLYCEROL DEHYDROGENASE AND NADH OXIDASE, Analytical sciences, 10(3), 1994, pp. 445-448
A flow injection analysis system for the determination of glycerol has
been developed which incorporates a Clark-type oxygen electrode and a
n enzyme reactor packed with Sepharose 4B on which glycerol dehydrogen
ase and NADH oxidase are co-immobilized. The response of the system de
pended on the concentration of flavine adenine dinucleotide (FAD) adde
d into the carrier solution [0. 1 M glycylglycine-KOH buffer (pH 9.2)
containing 30 mM ammonium sulfate]. A linear relationship between the
glycerol concentration and the response current in the presence of 60
muM FAD was observed over the 0.1 - 1.5 mM range. The sampling frequen
cy was about 25 samples/h at a flow rate of 0.5 ml/min,and the relativ
e standard deviation was 0.65% at 2.5 mM glycerol level (n=10). Applic
ation of this system to commercial white and red wines gave satisfacto
ry results compared with the F-kit method. No sample pretreatment was
necessary other than dilution.