CALCIUM-ACTIVATED POTASSIUM CHANNEL IN GROWTH-PLATE CHONDROCYTES - REGULATION BY PROTEIN-KINASE-A

Single channel K+ currents were recorded from inside-out patches of me mbrane obtained from cultured chicken growth plate chondrocytes. With a symmetrical 140 mM concentration of K+ across the cell membrane, a l arge conductance (21 1 pS), outward-rectifying, K+ channel was identif ied. Opening of this channel was dependent on the presence of internal Ca2+ with half maximal activation of the channel occurring with 3 mu M Ca2+. The channel was blocked by internal application of tetraethyla mmonium (IC50 = 45 mM) and 1 mM Ba2+. Application of the catalytic sub unit of protein kinase A to the cytosolic membrane resulted in over a three-fold increase in the open probability for the channel. Thus, gro wth plate chondrocytes contain a large-conductance, Ca2+-activated Kchannel which can be up-regulated during stimulation of protein kinase A. (C) 1994 Academic Press, Inc.