Rl. Mynatt et al., CHANGES IN CARNITINE PALMITOYLTRANSFERASE-I MESSENGER-RNA ABUNDANCE PRODUCED BY HYPERTHYROIDISM AND HYPOTHYROIDISM PARALLEL CHANGES IN ACTIVITY, Biochemical and biophysical research communications, 201(2), 1994, pp. 932-937
To study the regulation of carnitine palmitoyltransferase-I by thyroid
hormone, a cDNA was obtained by PCR amplification of DNA obtained by
reverse transcription of rat liver RNA CPT-I mRNA abundance was measur
ed in livers of hyperthyroid, euthyroid and hypothyroid rats. In hypot
hyroid rats, the CPT-I mRNA levels decreased 40-fold relative to that
of the hyperthyroid animals. These changes paralleled alterations in e
nzyme activity. These data suggest that CPT-I is regulated at the tran
scriptonal level by thyroid hormone. (C) 1994 Academic Press, Inc.