CHANGES IN CARNITINE PALMITOYLTRANSFERASE-I MESSENGER-RNA ABUNDANCE PRODUCED BY HYPERTHYROIDISM AND HYPOTHYROIDISM PARALLEL CHANGES IN ACTIVITY

To study the regulation of carnitine palmitoyltransferase-I by thyroid hormone, a cDNA was obtained by PCR amplification of DNA obtained by reverse transcription of rat liver RNA CPT-I mRNA abundance was measur ed in livers of hyperthyroid, euthyroid and hypothyroid rats. In hypot hyroid rats, the CPT-I mRNA levels decreased 40-fold relative to that of the hyperthyroid animals. These changes paralleled alterations in e nzyme activity. These data suggest that CPT-I is regulated at the tran scriptonal level by thyroid hormone. (C) 1994 Academic Press, Inc.