S. Aono et al., CLONING AND EXPRESSION OF THE GENE ENCODING THE 7FE TYPE FERREDOXIN FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII, Biochemical and biophysical research communications, 201(2), 1994, pp. 938-942
The structural gene (fdxA) coding for a 7Fe type ferredoxin of a oxidi
zing bacterium Bacillus schlegelii has been cloned and thermophilic hy
drogen sequenced. The fdxA coding region is 231 nucleotides which code
s for a 77 amino acids protein with the molecular weight of 8,744 exce
pt for iron-sulfur dusters. The fdxA gene has been expressed in E. col
i to obtain the recombinant ferredoxin. The recombinant ferredoxin hav
e shown the identical properties to the native one for absorption spec
tra, and SDS-PAGE. (C) 1994 Academic Press, Inc.