CLONING AND EXPRESSION OF THE GENE ENCODING THE 7FE TYPE FERREDOXIN FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII

Authors
AONO S NAKAMURA S AONO R OKURA I
Citation
S. Aono et al., CLONING AND EXPRESSION OF THE GENE ENCODING THE 7FE TYPE FERREDOXIN FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII, Biochemical and biophysical research communications, 201(2), 1994, pp. 938-942
Citations number
15
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
201
Issue
2
Year of publication
1994
Pages
938 - 942
Database
ISI
SICI code
0006-291X(1994)201:2<938:CAEOTG>2.0.ZU;2-N
Abstract
The structural gene (fdxA) coding for a 7Fe type ferredoxin of a oxidi zing bacterium Bacillus schlegelii has been cloned and thermophilic hy drogen sequenced. The fdxA coding region is 231 nucleotides which code s for a 77 amino acids protein with the molecular weight of 8,744 exce pt for iron-sulfur dusters. The fdxA gene has been expressed in E. col i to obtain the recombinant ferredoxin. The recombinant ferredoxin hav e shown the identical properties to the native one for absorption spec tra, and SDS-PAGE. (C) 1994 Academic Press, Inc.