MOLECULAR-CLONING AND CHARACTERIZATION OF MURINE CYCLIN-E

Using a monoclonal antibody developed to a mouse trophectodermal carci noma stem cell line E6496D lysate, we have identified the gene that en codes murine cyclin E. The cDNA contains a consensus cyclin box and a long 3' untranslated region and shares over 75% homology with human cy clin E cDNA. We show that the gene is expressed in fetal tissues, embr yonal carcinoma F9 cells and yolk sac carcinoma PYS-2 cells, but is no t expressed in preimplantation stages of development. In adult tissues , cycE mRNA is detectable in the spleen and to a lesser extent in the testis and brain. These data show that cycE is developmentally regulat ed and unevenly expressed in fetal and adult mouse tissues. (C) 1994 A cademic Press, Inc.