A SOLANUM (+) POTENTILLA INTERFAMILY PROTOPLAST REGENERANT WITH SOLANUM CHARACTERISTICS BUT WITH A POTENTILLA PLASTOME FRACTION

A Potentilla-specific plastome fraction in a predominantly Solanum-typ e regenerant from a protoplast co-culture was detected by RFLP. Fifty variegated lines from mixed protoplast populations of wildtype Potenti lla caulescens and a Solanum hybrid containing albino-mutant potato pl astids were studied. The nuclear genome of all lines was uniparental S olanum, as indicated by morphology, isozyme patterns and chromosome nu mber. Heterofamily plastid DNA was detected in one line by using 6 pla stid DNA probes of barley. All of the Solanum restriction fragments we re recovered. Additionally, probes of the psbA gene and 16SrDNA reveal ed Potentilla-specific signals, and an ndhE+G gene probe detected a no nparental fragment. Only Solanum-type fragments hybridized with the ps bA gene probe in albino sublines. Protoplast-derived clones were eithe r albinic or variegated. I is proposed that the line represents a cybr id with recombinant Solanum (+) Potentilla plastid DNA transmitted in combination with the entire Solanum plastid DNA.