FLUORESCENCE REAGENTS FOR LABELING OF BIOMOLECULES .2. REACTIONS OF 9-ISOTHIOCYANATOACRIDINE WITH AMINO-ACIDS

N-(9-Acridinylthiocarbamoyl)amino acids (ATC-AA) II - VII were synthes ized by reaction of amino acids with 9-isothiocyanatoacridine I, a new fluorescence labelling agent. The amino acid derivatives II - VII sho w high relative fluorescence, which is suitable for the determination of nanomolar amounts of ATC-AA. The kinetic measurements show that rea ction of I with amino acids is 6 to 22 times faster than analogous rea ction of phenyl isothiocyanate. The possibility of using 9-isothiocyan atoacridine for structure determination of proteins is discussed.