A FOCUSING ALGORITHM FOR HIGH MAGNIFICATION CELL IMAGING

An algorithm to produce a uniformly focused image in digital acquisiti on of high magnification light microscopy images is presented, In Very high magnification microscopic imaging the specimen surface cannot be considered ideally fat so that capturing a single image frame is usua lly not sufficient to capture an image that is focused everywhere. An image formation model for light microscopic images is presented, and b ased on this model an algorithm to construct a uniformly focused image is presented, The algorithm requires that multiple frames of the imag e at different focal planes be processed to combine their information to obtain an estimated of the desired image which is more completely f ocused than any of the individual frames. Experimental results show th at the proposed algorithm is very effective in approximating the desir ed image in high magnification microscopic imaging and highly robust c omparing to the gradient method.